In-vitro Estimation of Phytochemicals & Flavonoids in Citrus sinensis & Study their Antimicrobial & Antioxidant Activity
A. Kumar *
I.K.G. Punjab Technical University, Jalandhar, Punjab, India.
A. Mahajan
SUS. College of Engineering & Technology, Mohali (Punjab), India.
Z. Begum
SUS. College of Engineering & Technology, Mohali (Punjab), India.
*Author to whom correspondence should be addressed.
Abstract
Orange (C. sinensis) peel flavonoids have antibacterial, antioxidant, anticancer, anti-inflammatory, and antiviral effects. However, there is a scarcity of information in the literature about orange peels flavonoids. So, we estimated phytochemicals in hydro-methanolic extract of C. sinensis peel and flavonoids were separated by Column chromatography and confirmed with TLC and HPLC. After that we evaluated the antimicrobial, antioxidant and nitric oxide scavenging activity. The MIC value of flavonoids against E. coli, B. subtilis, S. aureus and P. aeruginosa is 19.40, 20.48, 12.45 and 21.44µg respectively. The IC50 value of flavonoids for antioxidant and Nitric oxide scavenging activity is 121.4 and 0.88µg respectively. In the current study, Citrus peel flavonoids were found to have antibacterial, antioxidant, and nitric oxide scavenging action in methanolic extracts. This study suggests that C. sinensis flavonoids could be exploited as an antibacterial and antioxidant source in the future.
Keywords: Citrus sinensis, flavonoids, antimicrobial activity, MIC, antioxidant and nitric oxide scavenging activity